Clostridium perfringens isolate typing by multiplex PCR
نویسندگان
چکیده
منابع مشابه
Typing of Toxigenic Isolates of Clostridium perfringens by Multiplex PCR in Ostrich
Clostridium perfringens is an important pathogen that provokes numerous different diseases. This bacterium is classified into five various types, each of which capable of causing a distinct disease. There are various methods for the bacterial identification, many are labor-intensive, time-consuming, expensive and also show low sensitivity and specificity. The aim of this research was to identif...
متن کاملTyping of isolates of Clostridium perfringens from healthy and diseased sheep by multiplex PCR
In this study, C. perfringens strains isolated from healthy and diseased sheep were analysed by multiplex PCR in order to to detect the presence of the alpha, beta, epsilon, iota and enterotoxin genes. C. perfringens was isolated from 52 of 104 sheep with enterotoxemia signs and from 61 of 194 clinically healthy sheep. Genotyping of 52 strains from diseased sheep indicated that 33 (64%) were ty...
متن کاملEvaluation of two PCR-based procedures for typing Clostridium perfringens.
Two polymerase chain reaction (PCR)-based procedures for typing Clostridium, perfringens, which affects most domestic animals, were compared and evaluated for efficiency as substitute to the guinea-pig intradermal test routinely used in our laboratory, namely a multiplex PCR and a protocol based on the individual amplification of gene sequences specific for each toxin. Reference isolates of C. ...
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Clostridium perfringens and C. difficile have been associated with acute and chronic large and small bowel diarrhoea, and acute haemorrhagic diarrhoeal syndrome in dogs. The objective of this study was to investigate by toxin gene profile and PCR-ribotyping the molecular characteristics of 14 C. perfringens and 10 C. difficile isolates from 95 canine faeces (n = 36, diarrhoeic and n = 59, non-d...
متن کاملtyping of clostridium perfringens isolated from broiler chickens using multiplex pcr
clostridium perfringens (c. perfringens) is related to gram-positive and anaerobic bacteria. this rod-shaped bacterium produces spores and can cause a wide spectrum of diseases in humans and animals. every of five different bacterium types can lead to different diseases. most of distinct bacterial recognition methods are not completely effective and practical in all condition. the objective of ...
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ژورنال
عنوان ژورنال: Journal of Venomous Animals and Toxins including Tropical Diseases
سال: 2010
ISSN: 1678-9199
DOI: 10.1590/s1678-91992010000400006